Figure 2
From: JNK–NQO1 axis drives TAp73-mediated tumor suppression upon oxidative and proteasomal stress
WA induces ROS and anti-oxidant response in tumor cells to trigger cell death. (a) DCF-DA-stained H1299 cells show elevated ROS levels upon WA treatment, which was prevented by NAC pretreatment. (b) ROS scavenger NAC abrogates WA-induced growth suppression in H1299 and HCT116TP53−/− cells. (c) WA-induced ROS upregulates protein levels of TAp73 and PUMA along with NRF2 and its anti-oxidant effectors NQO1 and HO-1. (d) ROS induced the expression of anti-oxidant response genes NFE2L2, NQO1 and HMOX-1 whereas NAC pretreatment reversed this effect. (e) Immunoblots of WA-treated HCT116TP53−/− cells show induced TAp73 protein levels, which correlated with the induction of NRF2, NQO1, HO-1, PUMA, Bid and PARP. This effect was prevented by NAC pretreatment for 8 h that is also apparent from (f) qPCR analysis
