Figure 4
From: Impact of conditional deletion of the pro-apoptotic BCL-2 family member BIM in mice
Induced deletion of Bimfl in adult animals renders thymocytes resistant to BIM-dependent apoptotic stimuli. Thymocytes from 4-OHT-treated Bimfl/fl/Vav-CreER-tg, Bimfl/fl/Rosa-CreER-tg and control mice were cultured (a) in medium only, or were (b) treated with 1 μg/ml Ionomycin (Sigma) or (c) with 2 ng/ml PMA (Sigma). At the indicated time points, the percentages of live cells were determined by staining with AnnexinV/PI (AnnexinV-PI=living cells). N=3–7 mice for each genotype. Data represent mean±S.E.M. *P<0.05, **P<0.01, ***P<0.001 (paired t-test)
