Figure 2

TRAF1 participates in the regulation of liver I/R injury. (a) Western blot analysis of TRAF1 expression in liver tissues from TRAF1-KO and WT mice. (b, h) Representative images of the areas of focal necrosis in hepatic cells of the indicated mice at different time points after I/R (left). Scale bar: 100 μm. Quantification of the necrotic areas (right; n=8 at each time point, *P<0.05 versus WT or NTG). (c, i) Quantification of serum marker ALT and AST levels at the indicated time points after I/R (n=8 at each time point, *P<0.05 versus sham, ^#P<0.05 versus I/R). (d) Schematic representation of two transgene constructs. (e) Representative images of liver I/R injury and quantification of necrotic areas (n=8, ^#P<0.05 versus NTG). (f) Quantification of serum ALT and AST levels. (g) Western blot analysis of TRAF1 detection in hepatic tissues from four founder lines of TRAF1 TG mice (TG1–TG4) and NTG mice and relative TRAF1 levels in TG1–TG4 and NTG mice (the number on the bar represents the fold -change). Data are presented as the mean±S.D.