Figure 5
From: TRAF1 is a key mediator for hepatic ischemia/reperfusion injury
TRAF1 is a modulator of hepatocyte survival after H/R. (a) Western blot analysis of TRAF1 expression in cultured hepatocytes infected with the indicated adenovirus (left) and the quantification of TRAF1 normalized to GAPDH. (n=4, *P<0.05 versus AdshRNA, #P<0.05 versus AdGFP). (b) Cell viability, LDH release and DNA fragmentation of hepatic cells isolated from WT/TRAF1 KO and NTG/TRAF1 TG mice at the indicated time points after H/R. (n=11 or 12 for each time point, *P<0.05 versus Basal, #P<0.05 versus H/R). (c) Cell viability, LDH release and DNA fragmentation of primary hepatocytes infected with AdshRNA/AdshTRAF1 and AdGFP/AdTRAF1 at the indicated time points after H/R (n=12 for each time point, *P<0.05 versus Basal, #P<0.05 versus H/R). (d, e) Western blot analysis of apoptosis-related protein levels in cells infected with the indicated adenoviral vectors for 48 h and subjected to H/R for 6 h. Right panel: quantification of protein levels (n=6, *P<0.05 versus AdshRNA or AdGFP control, #P<0.05 versus AdshRNA or AdGFP H/R). Data are presented as the mean±S.D.
