Figure 7

bmf-Deficiency confers protection against ischemic injury. Infarct volume was assessed 24 h after focal cerebral ischemia by cresyl violet/Nissl staining. Infarct volume was calculated for direct infarct volume and corrected for edema of infarcted tissue for (a) total indirect infarct volume and (b) indirect infarct volume of each section. Lower representation of infarct volume in mice deficient in bmf following tMCAO was not found to be significantly reduced when compared with WT mice (Mann–Whitney U test). Results were quantified and presented as a percentage of infarct volume compared with WT-treated mice. (c and d) Neurological deficit scores after induction of tMCAO in WT and bmf-deficient mice at 0 and 24 h following 60 min ischemia with reperfusion. Mice deficient in bmf score were significantly better than WT matched controls after 24 h reperfusion (Fisher’s exact test). (e) Neurological deficit scores at 24 h following 60 min ischemia with reperfusion with inclusion of two WT mice that demonstrated stroke-related mortality before 24 h. The bmf- deficient mice maintained a significant reduced deficit compared with WT controls after 24 h reperfusion. Circles and bar represent deficit score and median score, respectively; *P<0.05 compared with WT control. Data are representative of n≥12–13 for each group of WT and bmf-deficient mice