Figure 3 | Cell Death & Disease

Figure 3

From: Glucose transporter 1-mediated glucose uptake is limiting for B-cell acute lymphoblastic leukemia anabolic metabolism and resistance to apoptosis

Figure 3

Glut1 deletion suppresses glucose contribution to anabolic pathways and increases catabolic metabolism. (a) 13C-glucose tracing contribution of glucose to indicated metabolite pools. Graphs on the left indicate the total quantity of each metabolite and the graph on the right indicates the relative distribution of 13C carbons in each metabolite. (b and c) WT Cre-ER and Glut1fl/fl Cre-ER B-ALL cells were treated with vehicle or 4-OHT for 96 h followed by 48 h of culture without 4-OHT and (b) pentose phosphate pathway flux was measured by oxidation of 1-C14-glucose and (c) lipid oxidation was measured by oxidation of C14 palmitate. Means and S.D. of triplicate measurements are shown. *P<0.05, **P<0.005, ***P<0.001, ****P<0.0001. NS, not significant

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