Figure 3 | Cell Death & Disease

Figure 3

From: Identification and characterization of the novel Col10a1 regulatory mechanism during chondrocyte hypertrophic differentiation

Figure 3

Y1H screening using the 150-bp enhancer as a bait. (a) The 150-bp Col10a1 cis-enhancer (tandem copies, 2 × 150) was cloned upstream of the nutritional reporter gene HIS3 in a pHis2.1 vector backbone (Clontech). (b) After screening a cDNA library derived from hypertrophic MCT cells, approximately 50 yeast colonies grown in selective medium and optimal 3-AT concentration were selected and subjected to PCR amplification. (c) Representative sequencing result of the PCR product shows partial sequence of exon II of gene Anxa2. (d) A list of candidate genes identified by Y1H were shown. These genes include Fn1, Hspa5, Nedd4, Psmb1, Ptgs2, Rab1, Rela, Rpl35 and Rps28. The functional description and relevant literatures were as listed

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