Figure 2

Shh signaling is upregulated in the ischemic cortex at 9 h after MCAO. Neurons (a) in the ischemic area and (b) in the contralateral cortex express Shh. (c) Some GFAP+ astrocytes (arrow) and endothelial cells (arrow head) express Shh at 9 h after stroke. (d) Neurons in the infarct area express Shh receptor Ptch1. Ptch1+ astrocytes present in the (e) ischemic parenchyma and (f) perivascular units. (g and h) Neurons in the ischemic area express Shh co-receptor Smo. (i) Smo+ parenchymal (arrow) and perivascular (arrow head) astrocytes and (j) oligodendrocytes in the ischemic cortex. Vascular endothelial cells expressing (k) Smo and (l) Smo-negative endothelial cells in the ischemic area. Scale bar, 50 μm for e and i; 20 μm for (c, d, f–h, k and l) 10 μm for (a, b and j). Cx, cortex