Figure 3 | Cell Death & Disease

Figure 3

From: A Smoothened receptor agonist is neuroprotective and promotes regeneration after ischemic brain injury

Figure 3

Effect of PUR on Shh signaling and inflammation. Level of mRNA coding for (a) Shh, (b) Ptch1, (c) Smo and (d) Gli2 in SHAM- (n=6), VEH- (n=11) and PUR-treated animals (n=11) at 9 h after MCAO. (e) Gli1 mRNA level at 9 and 24 h (n=6) after stroke. (f) Immunoblot analysis of Gli1 and Gli2 in the nuclear protein fraction isolated from ischemic cortex at 9 h after stroke (n=4–5). (g) Quantification of protein level normalized to loading control Lamin B1 (n=5). Bands cropped from the same blot are separated by a vertical line. mRNA expression level of pro-inflammatory cytokines (h) IL6, (i) IL1β and (j) TNFα detected at 9 (n=11) and 24 h (n=6) after MCAO in VEH- and PUR-treated animals. Data are the means±S.E.M.; *P<0.05; **P<0.01; ***P<0.001

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