Figure 4

PUR reduces apoptotic cell death in the ischemic cortex after MCAO. Apoptotic cells in the ischemic cortex of (a and c) VEH- and (b and d) PUR-treated animals at 9 and 24 h after MCAO (representative image of n=4–5; scale bar, 50 μm). Apoptotic neurons (insert in panel (a); scale bar, 20 μm). Cx, cortex. (e) Quantitative analysis of apoptotic cells in the ischemic cortex (n=4–5). Data are the means±S.E.M.; *P<0.05. (f) Representative blots for cleaved caspase-3, Bad, Bax, Bcl-2 and Bcl-xL in the cytoplasmic and AIF in the nuclear protein fraction. Bands cropped from the same blot are separated by a vertical line. (g and h) Quantification of protein levels relative to loading control β-actin. Data are the means±S.E.M.; n=6; *P<0.05