Figure 5
PUR increases the expression of tPA in ischemic neurons early after treatment. Levels of mRNA coding for (a) Bcl-2, (b) brain-derived neurotrophic factor (BDNF), (c) nerve growth factor (NGF), (d) vascular endothelial growth factor (VEGF) and (e) Plat at 9 (n=6–11) and 24 h (n=6) after MCAO. (f) Immunoblot analysis of tPA protein level and enzymatic activity after stroke. Quantification of (g) tPA protein level and (h) tPA activity normalized to β-actin (n=6). Bands cropped from the same blot are separated by a vertical line. Data are the means±S.E.M.; *P<0.05; **P<0.01; ***P<0.001. (i) tPA+ neurons in the ischemic cortex at 9 h after MCAO in VEH- (arrows) and (j and k) PUR-treated animals (representative images of n=4). (l) Parenchymal astrocytes in the ischemic cortex did not express tPA at 9 h after stroke (m) while some perivascular astrocytes were tPA+. Scale bar, 20 μm for I-L; 10 μm for M. Cx, cortex. (n) BBB permeability at 10 h after MCAO. Data are the means of n=5±S.E.M.; *P<0.05; ***P<0.001
