Figure 6
PUR reduces astrogliosis and inflammation at a later time point after stroke. Reactive astrocytes in the glial scar express (a) Shh, (b) Ptch1 and (c) Smo. Representative images of n=8–10. mRNA expression levels of (d) Shh, (e) Ptch1, (f) Smo, (g) Gli1 and (h) GFAP in the ischemic cortex at 7 and 14 d after stroke (n=6–7). Expression of GFAP in the glial scar at 14 d after MCAO in (i) VEH- and (j) PUR-treated animals. Representative images of n=8–10. (k) Mean GFAP intensity in the glial scar in areas corresponding to grey (GM1) or white (WM1) matter in the peri-infarct zone and areas located at 300 μm from the border to the ischemic zone (GM2, WM2; n=8–10). Levels of mRNA coding for (l) CD11b, (m) CD45, (n) TNFα, (o) IL6 and (p) IL1β at 7 and 14 d after stroke (n=6–7). Data are the mean±S.E.M.; *P<0.05; **P<0.01; ***P<0.001. Scale bar, 50 μm for a–c, i and j. Cx, cortex; NS, not significant
