Figure 3
Confocal microscopy analyses of subcellular localization of ANG. (a and b) Vector control, (c and d) WT-APOA1, and (e and f) L75P-APOA1 transfectants of HepG2 cells were cultured in normal growth medium (a, c, e) or in serum-free medium (b, d, f), fixed, and stained by ANG mAb 26–2F and Alexa 610-labeled goat anti-mouse F(ab’)2. Nuclei were stained with Hoechst 33342. Confocal images were taken with a Leica TCS SP5 confocal microscope. The images shown are the middle panels of the Z-sections. Nucleolar and cytoplasmic ANG are indicated by arrows and dashed arrows, respectively. Scale bar: 20 μm. DAPI, 4,6-diamidino-2-phenylindole
