Figure 5
Effect of L75P-ApoA-I on cell apoptosis. (a–d) The vector control, (e–h) WT-APOA1, and (i–l) L75P-APOA1 transfectants were cultured in normal growth medium (a, b, e, f, i, j) or in serum-free medium (c, d, g, h, k, l) in the presence (b, d, f, h, j, l) or absence (a, c, e, g, i, k) of 0.5 μg/ml ANG for 48 h. Cells were collected and stained with EB (red) and AO (green). EB-stained cells were counted in a total of 750 cells from five randomly selected areas of each slide. The percentage of apoptotic cells is shown at the bottom of each image. Data shown are the means±S.E.M. of triplicates of a representative experiment of at least three repeats
