Figure 7
From: PGC-1α buffers ROS-mediated removal of mitochondria during myogenesis

Depletion of PGC-1α inhibits myogenin and induces FBXO32 expression. C2C12 cells were transfected with scramble (scr) or PGC-1α siRNA (PGC-1α(−)) and differentiated in DM for the indicated days. (a, b) Total RNA was isolated, and relative mRNA levels of MYOG (a) and FBXO32 (b) were analyzed by RT-qPCR. Data are expressed as means±S.D. (n=4, *P<0.001 versus day 0 scr cells; °P<0.001 versus scr cells). (c, d) C2C12 cells were transfected with scramble (scr), PGC-1α and/or PINK1 siRNA (PGC-1α(−), PINK1(−) and PGC-1α/PINK1(−)) and differentiated for 2 days. Total RNA was isolated, and relative mRNA levels of MYOG (c) and FBXO32 (d) were analyzed by RT-qPCR. Data are expressed as means±S.D. (n=4, *P<0.001 versus scr cells; °P<0.001 versus PGC-1α(−) cells, PGC-1α(−)/PINK1(−) cells and #P<0.001 versus PINK1(−) cells)