Table 1 List of primers used for RT-qPCR and ChIP analysis
From: PGC-1α buffers ROS-mediated removal of mitochondria during myogenesis
Gene | Sequences |
|---|---|
COX4I1 (mouse) | RV 5′-TCACAACACTCCCATGTGCT-3′ FW 5′-GAATGTTGGCTTCCAGAGCG-3′ |
PGC-1α (mouse) | RV 5′-ACTCGGATTGCTCCGGCCCT-3′ FW 5′-ACTGACGGCCTAACTCCACCCA-3′ |
MT-CO1 (mouse) | RV 5′-ACCTATTCGTACTCCGGCCT-3′ FW 5′-ATGTGACCCGATTTGGCGTT-3′ |
MT-ATP6 (mouse) | RV 5′-GTGGAAGGAAGTGGGCAAGTGAGC-3′ FW 5′-GCCATTCCACTATGAGCTGGAGCC-3′ |
TFAM (mouse) | RV 5′-TCCGCCCTATAAGCATCTTG-3′ FW 5′-CCGAGGTGGTTTTCATCTGT-3′ |
PARK2 (mouse) | RV 5′-ACAGGGCTCCTGACATCTG-3′ FW 5′-CAAGGACACGTCGGTAGCTT-3′ |
LC3 (mouse) | RV 5′-CCACTCTTTGTTCAAAGCTCCGGC-3′ FW 5′-CGTCGCCGGAGTCAGATGTC-3′ |
NRF1 (mouse) | RV 5′-ATGGGCGGCAGCTTGACTGT-3′ FW 5′-GCGCAGCCGCTCTGAGAACTTAT-3′ |
NRF2 (mouse) | RV 5′-TGGGCCCTGATGAGGGGCAGTG-3′ FW 5′-TCCGCCAGCTACTCCAGGTTGG-3′ |
POLRMT (mouse) | RV 5′-GACGGCGTTAGGTTGACTGA-3′ FW 5′-CCAGTTCACCAGGATGGCTC-3′ |
POLG (mouse) | RV 5′-ACAAGTCCTCGGTCCGCTTC-3′ FW 5′-CACCCTGAGGCTGCGTG-3′ |
RPL (mouse) | RV 5′-ATGGCGGAGGGGCAGGTTCTG-3′ FW 5′-GTACGACCACCACCTTCCGGC-3′ |
BNIP3 (mouse) | RV 5′-GGTCGACTTGACCAATCCCAT-3′ FW 5′-ACAGCACTCTGTCTGAGGAA-3′ |
D-Loop consensus sequence | RV 5′-CATGAATAATTAGCCTTAGGTGAT-3′ FW 5′-TCAGACATCTGGTTCTTCTTACTTCAG-3′ |
FOXO1 consensus sequence on LC3 promoter | RV 5′-CCCAAGGATCTCAACCAAAC-3′ FW 5′-CCTCAGCTGGTAAGAGCAT-3′ |
FOXO1 consensus sequence on PINK1 promoter | RV 5′-CTGTCGACCGCCATGGTGGCGCGGTGACC-3′ FW 5′-TGAGAGCACTTGGGAGTGGGGGAGAAGAG-3′ |
