Figure 1
Expression and cellular localization of CDK1 in hESC. (a) Western blot of a total hESC lysates probed with antibodies against total CDK1 and specific phospho-residues important for cell cycle progression. Cells were synchronized at G1 (70%), S (75%) and G2 (68%) phases of the cell cycle as described before at Neganova et al.;2 H9-unsynchronized population. (b) Analysis of specific complex formation between CDK1/Cyclin B1 and CDK1/Cyclin A via immunoprecipitation in unsynchronized hESC. (c) CDK1 cellular localisation in H9 hESC during transition from late G1 to S phase and late S/G2 phase transition revealed by indirect immunofluorescence with anti-CDK1 antibody (green) and anti-Ki67 (red). Scale bar=10 μm. Presented images are representative of at least three independent experiments
