Table 1 The sequences of oligonucleotides used for qRT-PCR analysis.
Gene Name | Primer (5’-3’) |
|---|---|
CDK1 | For: TTTTCAGAGCTTTGGGCACT Rev: CCATTTTGCCAGAAATTCGT |
OCT4 | For: AGCTCTGCAGAAAGAGTCCCAGG Rev: TGAGCCCCACATCGGCCTGT |
KLF4 | For: CCCAATTACCCATCCTTCCT Rev: CGTCCCAGTCACAGTGGTAA |
Lin28 | For: TCCTGCACTGTGTTCTCAGG Rev: AAAGCCAGCTCTTATTGGCA |
SOX2 | For: GGCAGCTACAGCATGATGCAGGACC Rev: CTGGTCATGGAGTTGTACTGCAGG |
NANOG | For: TCCAGCTTGTCCCCAAAGCTTGC Rev: ACAGTCTCCGTGTGAGGCATCT |
CDX2 | For: GGCAGCCAAGTGAAAACCAG Rev: GGTGATGTAGCGACTGTAGTGAA |
GATA4 | For: ACACCCCAATCTCGATATGTTTG Rev: GTTGCACAGATAGTGACCCGT |
PAX6 | For: ACAGTCACAGCGGAGTGAATC Rev: ACTTTTGCATCTGCATGGGTC |
FGF5 | For: ATTTGCTGTGTCTCAGGGGAT Rev: CTGTGAACTTGGCACTTGCAT |
NESTIN | For: CAGGAGAAACAGGGCCTACA Rev: TGGGAGCAAAGATCCAAGAC |
BRACHYURY | For: TCAGCAAAGTCAAGCTCACCA Rev: CCCCAACTCTCACTATGTGGATT |
AFP | For: CTTTGGGCTGCTCGCTATGA Rev: ATGGCTTGGAAAGTTCGGGTC |
GAPDH | For: TGCACCACCAACTGCTTAGC Rev: GGCATGGACTGTGGTCATGAG |
RPL12A | For: CCTGGAGGAGAAGAGGAAAGAGA Rev: TTGAGGACCTCTGTGTATTTGTCAA |
SDHA | For: TGGGAACAAGAGGGCATCTG Rev: CCACCACTGCATCAAATTCATG |
