Figure 2 | Cell Death & Disease

Figure 2

From: A sustained deficiency of mitochondrial respiratory complex III induces an apoptotic cell death through the p53-mediated inhibition of pro-survival activities of the activating transcription factor 4

Figure 2

The time-dependent effect of mitochondrial ETC complex III inhibition on the expression of ATF4 and its target genes in HCT116 cells. (a–f) The effects of myxothiazol (1 μM) for indicated intervals of time on ATF4, CHOP, TRIB3, SLC7A11, CHAC1 and TP53INP1 mRNA levels in HCT116 cells were examined by RT-qPCR. Mean and S.D. are presented of three independent experiments. All values are normalized to the level of the corresponding mRNA in the control (untreated) cells. (g) Western analysis of p53 in myxothiazol-treated HCT116 cells for indicated intervals of time

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