Figure 5 | Cell Death & Disease

Figure 5

From: A sustained deficiency of mitochondrial respiratory complex III induces an apoptotic cell death through the p53-mediated inhibition of pro-survival activities of the activating transcription factor 4

Figure 5

Preliminary p53 activation prevents the upregulation of ATF4 and its target genes in response to short respiration chain inhibition. (a) Western analysis of p53 in Nutlin-3 (16 h)/myxothiazol (4 h) treated HCT116 wt and p53 −/− cells. (b–c) The effects of Nutlin-3 (16h) and/or myxothiazol (4 h) on TP53INT1 and ATF4 mRNA levels in HCT116 wt and p53 −/− cells were examined by RT-qPCR. Mean and S.D. are presented of three independent experiments. (d–e) The effects of myxothiazol (4 h) or Nutlin-3 (16 h) and myxothiazol (4 h) on ATF4, ASNS, SLC7A11 and TRIB3 mRNA levels were examined by RT-qPCR in HCT116 wt cells (d) and HCT116 p53 −/− cells (e). Mean and S.D. are presented of three independent experiments. Student’s t-test was used to analyze statistical significance (*P<0.05, **P<0.01, ***P< 0.001). All values are normalized to the level of the corresponding mRNA in the control (untreated) cells

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