Figure 1 | Cell Death & Disease

Figure 1

From: Cancer cell death induced by novel small molecules degrading the TACC3 protein via the ubiquitin–proteasome pathway

Figure 1

SNIPER(TACC3) decreases the TACC3 protein level. (a) Chemical structure of SNIPER(TACC3). (b) HT1080 cells were treated with the indicated concentration of Me-BS, SNIPER(TACC3)-1, SNIPER(TACC3)-2 or KHS108 for 6 h (upper panels) or 24 h (lower panels). (c) MCF7 and U2OS cells were treated with 30 μM of Me-BS, SNIPER(TACC3)-1, SNIPER(TACC3)-2 or KHS108 for 6 h. (d) HT1080 cells were treated with 30 μM of Me-BS, SNIPER(TACC3)-1, KHS108 or Me-BS plus KHS108 for 6 h. (e) MCF7 cells were treated with 10 μM of SNIPER(TACC3)-1 or SNIPER(CRABP)-4 for 6 h (left panels), or cells precultured in the media containing estrogen-depleted serum were treated with 10 μM of SNIPER(ER)-3 for 9 h (right panels). (b–e) Whole-cell lysates were analyzed using western blotting with the indicated antibodies. The numbers below the protein panels represent the protein level relative to control (dimethyl sulfoxide (DMSO)), which was normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or β-actin

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