Figure 2
From: CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling
Normal positive selection in CBAP-deficient thymocytes. (a) Total thymocytes from 4-week-old littermates were stained for CD8, CD4, TCRβ, and CD69. Histograms of TCRβ and CD69 on gated DP thymocytes are shown (n=4). (b,c) Surface staining of CD8 and CD4 on thymocytes from 4-week-old littermate OT-I (b) and female HY (c) mice. Upper panels: Flow cytometric profiles. Lower panels: Absolute numbers of total, DP, and CD8SP thymocytes of mice carrying each TCR transgene were calculated as total cell number times the percentage of each thymocyte subpopulation (n=5−7)
