Figure 3
From: CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling
Compromised negative selection in CBAP-deficient thymocytes. (a) Thymocytes from 4-week-old male HY littermates were stained for CD8, CD4, HY TCR, and PI. Upper panel: Flow cytometric profiles. Lower panel: Absolute numbers of total, DP, and CD8SP thymocytes from mice carrying the HY TCR transgene (n=7) were calculated as in Figures 2b and c. (b) Seven-week-old littermates were injected with 10 μg SEB or PBS (vehicle) on day 0, 2, and 4. Mice were killed on day 6. Isolated thymocytes were counted and stained for CD8, CD4, Vβ8, and Vβ6, and analyzed by flow cytometry. Numbers of Vβ8+ and Vβ6+ thymocytes in CD8SP (upper) and CD4SP (lower) populations were plotted as mean±S.D. (n=7). n.s., not significant. (c) Eight-week-old littermates were injected with 10 μg (n=4) or 20 μg (n=5) anti-CD3 Ab or PBS (n=5). Mice were killed after 40 h. Isolated thymocytes were counted, stained for CD8, CD4, and PI, and analyzed by flow cytometry. Numbers of total and DP thymocytes were plotted as mean±S.D.
