Figure 5
From: CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling
Reduced JNK phosphorylation in CBAP-deficient thymocytes upon TCR crosslinking. (a) Thymocytes were cultured on plates coated with the indicated Abs. After 24 h, specific TCR-induced apoptosis was analyzed by flow cytometry and calculated as in Figure 4c (n=4). (b) Thymocytes were stimulated by anti-CD3 (5 μg/ml) and anti-CD4 (5 μg/ml) Abs for the indicated times. Lysates were prepared and levels of unphosphorylated and phosphorylated JNK1/2, P38, and ERK1/2 proteins were analyzed by western blotting (upper panel). Quantitative results for pJNK1/2 expression are shown in the lower panel (n=3). (c) Thymocytes with or without JNK inhibitor SP600125 pretreatment (JI, 5 μM, 30 min before Ab crosslinking) were cultured and analyzed as in Figure 4c. Survival of DP thymocytes was plotted as mean±S.D. (n=4)
