Figure 6
From: CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling
Involvement of CBAP in TCR-induced signaling complex formation. Thymocytes were stimulated with anti-CD3 (5 μg/ml) and anti-CD4 (5 μg/ml) Abs for the indicated times. (a) Proteins were prepared from cell lysates, and unphosphorylated and phosphorylated proteins were detected by western blotting (n=3). Phosphorylation sites are indicated in parentheses. (b,c) Immunoprecipitation was performed using anti-LAT (b) or anti-PLCγ1 (c) Ab with IgG controls, and western blots were probed with Abs for PLCγ1, ZAP70, LAT, and GRB2 (n=4)
