Figure 1

Neuritin expression increases neurite outgrowth. (a and b) Confocal images of either control vector- or neuritin-expressing neurons that were co-immunostained with eGFP alone or eGFP/Flag (green), Tau-1 (a, red) or MAP2 (b, red). Scale bar, 100 nm. Insets are × 6.9 magnified views of designated regions. Total axonal length (c: Control, 1017.13±112.96 μm, n=19 neurons versus Neuritin, 2555.57±341.08 μm, n=26 neurons) and dendritic length (d: Control, 709.35±78.79 μm, n=23 neurons versus Neuritin, 1446.69±171.17 μm, n=30 neurons) of control- and neuritin-expressing neurons are depicted. (e) Sholl analyses for neurites visualized with Flag staining. Neuritin slowed down the decrement rate of crossing numbers (at 30 μm: Control, 5.64±0.76 versus Neuritin, 7.88±0.71, *P=0.0402; at 40 μm: 4.86±0.93 versus 7.13±0.58, *P=0.0415; at 60 μm: 3.57±1.06 versus 7.19±0.77, **P=0.0011; at 70 μm: 3.57±0.97 versus 6.94±0.91, **P=0.0053; at 80 μm: 2.64±0.88 versus 4.25±1.06, ***P=0.0004; at 90 μm: 2.64±0.82 versus 5.94±0.62, **P=0.0018; at 100 μm: 2.27±0.63 versus 5.71±0.68, **P=0.0018; at 110 μm: 1.79±0.55 versus 5.44±0.61, ***P=0.0009; at 120 μm: 1.91±0.51 versus 4.69±0.58, **P=0.0012). Statistical significance is expressed as *P<0.05; **P<0.01; ***P<0.001