Figure 2
From: Neuritin can normalize neural deficits of Alzheimer’s disease
Neuritin expression enhances maturation of spines. (a) Representative images of either control vector (left)- or neuritin (right)-expressing neurons immunostained with eGFP/Flag and (green, top) and simultaneously labeled monomeric DsRed (mDsRed, red, middle). Fluorescence images are merged (bottom). Scale bar, 10 μm. (b) Spine numbers per 10 μm are measured (b–1: Control, 9.73±0.83 versus Neuritin, 12.86±0.90) and categorized into their subtypes (b–2: Class I: Control, 1.85±0.18 versus Neuritin, 2.53±0.22; Class II: 3.88±0.43 versus 6.23±0.51; Class III: 3.20±0.36 versus 3.26±0.38; Class IV: 0.80±0.09 versus 0.84±0.12) from control vector- (n=31) and neuritin-expressing (n=34) neurons. (c) Pie charts showing the frequency in which each type of spines are observed. (d) Cumulative probability diagrams for spine head diameters (d–1) and spine lengths (d–2). Insets: averaged spine head diameters (Control, 0.86±0.1 μm versus Neuritin, 0.97±0.01 μm) or spine lengths (1.78±0.03 μm versus 1.79±0.02 μm) are depicted, respectively. For (c and d), 1345 spines from 31 control vector-expressing neurons and 2245 spines from 34 neuritin-expressing neurons are used. Statistical significance is expressed as *P<0.05 and ***P<0.001
