Figure 1
Expression of miR-194 was induced during osteoblast differentiation and reduced during adipocyte differentiation. (a) Schematic representation of the eight miRNAs predicted to target COUP-TFII-3′-UTR. (b-d) Mesenchymal C3H10T1/2 cells were cultured in osteogenic induction medium (OM) or adipogenic induction medium (AM) for 3 days. (b) Expression levels of COUP-TFII and miRNAs as determined by qRT-PCR (n=3). After normalizing against β-actin or sno234, and compared with that of growth medium (GM) control. Values represent mean±S.D. *, P<0.05. (c) Relative expression was calculated after normalization to β-actin or sno234 levels (n=3). Values represent mean±S.D. *, P<0.05 and **, P<0.01. (d) Western blotting analysis was performed for protein levels of COUP-TFII. β-actin was used as a loading control. (e, f) Primary BMSCs were cultured in OM or AM for 4 days. The expression levels of miR-194 and COUP-TFII were analyzed by qRT-PCR. Relative expression was calculated after normalization to β-actin or sno234 levels (n=3). Values represent mean±S.D. *, P<0.05 compared with growth medium cultures. (g) Western blotting analysis was performed for protein levels of COUP-TFII. β-actin was used as a loading control
