Figure 3

MiR-194 promotes osteoblasts differentiation. (a, b) Primary osteoblasts were cultured in OM for the indicated time period. Total RNA was isolated, and the expression levels of miR-194 and osteogenic markers were evaluated by qRT-PCR or RT-PCR, respectively (n=3). *, P<0.05 and **, P<0.01 compared with day 0 group. (c–f) C3H10T1/2 cells were transfected with miR-194 precursors or anti-miR-194 for 24 h, and then treated with OM for an additional 4-day period before performing RT-PCR (c, e) or Western blot analysis (d, f) with the indicated primers or antibodies. All data presented were independently repeated three times. (g, h) C3H10T1/2 cells were transfected with miR-194 precursor or anti-miR-194 for 24 h, and then treated with OM for an additional 7-day period before alizarin red staining was performed. A representative image of three independent experiments was shown