Figure 4
MiR-194 suppresses adipocytes differentiation. (a, b) 3T3-L1 cells were cultured in AM for the indicated time period, after which total RNA was isolated and expression levels of miR-194 and adipogenic markers were evaluated by qRT-PCR or RT-PCR, respectively (n=3). **, P < 0.01 compared with day 0 group. (c, e) 3T3-L1 cells were transfected with miR-194 precursors or anti-miR-194 for 24 h, and then cultured in AM for an additional 4-day period before performing RT-PCR with indicated primers, respectively (n=3). *, P<0.05 compared with the miR-CTL group. (d, f) Western blot analysis with indicated antibodies. (g, h) 3T3-L1 cells were transfected with miR-194 precursors or anti-miR-194 for 24 h and then treated with AM for an additional 7-day period before performing Oil Red O staining. A representative image of three independent experiments was shown
