Figure 5
MiR-194 can regulate osteogenesis and adipogenesis from mesenchymal cells. (a) C3H10T1/2 cells were co-transfected with miR-194 (10 nM) or miR-CTL (10 nM) and COUP-TFII expression vector without 3′-UTR (100 ng) or control pcDNA3 vector (100 ng), cultured in OM for 6 days, and then subjected to alizarin red staining. The levels of calcium deposition was quantified by using image J software (n=3). *, P < 0.05 compared with the control group. #, P < 0.05 compared with the indicated group. (b) Expression levels of osteogenic markers were determined by RT-PCR after culturing in OM for 4 days. (c) 3T3-L1 cells were co-transfected with miR-194 (40 nM) or miR-con (40 nM) and the COUP-TFII expression vector (100 ng) or control pcDNA3 vector (100 ng), cultured in AM for 6 days and then subjected to Oil Red O staining. The levels of oil drop was quantified by using image J software (National Institutes of Health, Bethesda, MD, USA) (n=3). *, P < 0.05 and **, P < 0.01 compared with the control group. #, P < 0.05 compared with the indicated group. (d) Expression levels of adipogenic markers were determined by RT-PCR. (e) Schematic diagram of miR-194-mediated regulation of osteoblast and adipocyte differentiation from MSCs
