Figure 1

Smad7 is overexpressed in human sporadic CRC. (a) Total proteins extracted from both tumoral (T) and nontumoral (NT) areas of two patients with sporadic CRC were evaluated for Smad7 expression by western blotting. β-actin was used as loading control. The figure is representative of seven separate experiments in which similar results were obtained. Right inset. Quantitative analysis of Smad7/β-actin protein ratio in total extracts of T and NT tissues taken from fourteen patients with sporadic CRC, as measured by densitometry scanning of western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean±S.E.M. of all experiments (n=14). (b) Smad7 immunostaining in specimens taken from tumoral (T) and non-tumoral (NT) areas of one patient with sporadic CRC. The figure is representative of six separate experiments in which sections of six CRC patients were analyzed. Staining with isotype control IgG is also shown. The scale bars are 100 μm