Figure 3

Effect of Smad7 AS on the in vivo formation of HCT-116-derived xenografts. (a) Representative images showing the uptake of fluorescent-conjugated Smad7 antisense oligonucleotide (AS) in HCT-116-derived xenografts induced in Rag1^−/− mice. Smad7 AS-positive cells were evaluated by immunofluorescence. The scale bars are 20 μm. One of three separate experiments is shown. (b) Representative histograms showing the volume of HCT-116-derived tumors taken from mice treated with either Smad7 AS or Smad sense oligonucleotide (S). Data indicate the mean±S.D. of two independent experiments in which at least six mice per group were analyzed. Lower panel: representative photographic images of xenografts developed in mice treated with either Smad7 AS or S. (c) Representative pictures of SMAD7- and PCNA-stained sections of xenografts taken from mice treated as indicated in b. Isotype control stainings are indicated. The scale bars are 20 μm. The scale bar in the inset is 10 μm. One of six separate experiments is shown