Figure 3
The protective action of osmotin against ethanol-induced apoptosis in primary cultures of fetal hippocampal neurons is evidenced by cellular levels of apoptotic marker proteins. (a) Cells were exposed for 24 h to normal growth medium containing no supplements (Control), or supplemented with ethanol (EtOH) and osmotin (Osm) as indicated. Shown are immunoblots of cell extracts that were fractionated by SDS-PAGE. The caspase-3 antibody used was capable of recognizing both pro- and cleaved caspase-3. However, only cleaved caspase-3 bands were visible in these samples. Blots were reacted with antibody to β-actin as loading control. (b) Shown is a quantitative analysis of the data in (a). Density values, normalized to actin signals, are expressed as mean±S.D. (n=5) and are expressed as arbitrary units. Indicated pairs are significantly different at P<0.05. The inset shows the average Bcl-2 to average Bax signal ratio across the treatments
