Figure 5
Levels of apoptotic marker proteins in developing rat brains suggest a protective action of osmotin in vivo against ethanol-induced apoptosis. P7 rat pups were injected with saline (−) or ethanol (EtOH; 4 mg/g b.w.) and again with osmotin (Osm; 15 μg/g b.w.) or saline 30 min later. Shown are immunoblots representing levels of the indicated apoptotic marker proteins in tissue extracts at 12 h after ethanol administration. β-Actin is taken as loading control in each case. The experiment was repeated three times and one representative experiment is shown. Quantification and statistical analysis of the complete data are shown in Supplementary Figures S2a and b
