Figure 6
Immunofluorescence analysis of cytochrome c and caspase-3 levels showing the in vivo neuroprotective action of osmotin against ethanol-induced apoptosis in developing rat brain. P7 rat pups were injected with saline (Control) or ethanol (EtOH; 4 mg/g b.w.) and again with osmotin (Osm; 15 μg/g b.w.) or saline 30 min later. Pups were killed at 12 h after ethanol injection for analysis. Shown are tissue sections from the CA1 region of the hippocampus (A) and the anterior cingulate cortex (B) that were double stained for cytochrome c (FITC-label, green) and caspase-3 (TRITC-label, red). Yellow color (merge) indicates colocalization. (a–c) magnification, × 20; (d–i) magnification, × 40; scale bar, 20 μm
