Figure 3

RSV attenuated t-BHP-induced mitochondrial dysfunction by inhibiting mtROS generation in HUVECs. Cells were transfected with Sirt3 siRNA as described in the Materials and Methods section. At 24-h post-transfection, cells were pretreated with RSV (0.1, 1 and 10 μM) for 2 h, washed, and then incubated with fresh medium in the presence or absence of t-BHP (80 μM) for an additional 4 h. (a) The mitochondrial O₂^•− levels were estimated using MitoSOX Red and the fluorescence values were read at an excitation wavelength of 510 nm and emission wavelength of 579 nm. (b) The bar charts show quantification of the mitochondrial O₂^•− levels expressed as the fold change relative to the control group. All results are presented as means±S.E.M. of at least three independent experiments. ^bP<0.01 versus the control group; ^cP<0.05, ^dP<0.01 versus the t-BHP-treated group; ^eP<0.05 versus RSV (10 μM) and t-BHP (80 μM) co-treated group with control siRNA transfection