Figure 4 | Cell Death & Disease

Figure 4

From: Resveratrol regulates mitochondrial reactive oxygen species homeostasis through Sirt3 signaling pathway in human vascular endothelial cells

Figure 4

RSV reduced mtROS generation by stimulating Sirt3-mediated mitochondrial enzyme activities and SOD2 deacetylation. Cells were transfected with Sirt3 siRNA as described in the Materials and Methods section. At 24-h post-transfection, cells were pretreated with different concentrations of RSV (0.1, 1 and 10 μM) for 2 h and then treated with or without t-BHP of 80 μM for an additional 4 h. (a–c) The enzyme activities of IDH2 (a), GSH-Px (b) and SOD2 (c) were determined using the corresponding assay kits, according to the manufacturer’s instructions. (d) After the indicated treatments, the cells were harvested and lysed to detect protein levels of ac-SOD2 by western blot analysis. All results are presented as means±S.E.M. of at least three independent experiments. aP<0.05, bP<0.01 versus the control group; cP<0.05, dP<0.01 versus the t-BHP-treated group; eP<0.05, fP<0.01 versus RSV (10 μM) and t-BHP (80 μM) co-treated group with control siRNA transfection

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