Table 1 Primer details
Primer | Forward (5′-3′) | Reverse (5′-3′) | Annealing temperatures (°C) |
|---|---|---|---|
Atp 6 | GCCGCAGTACTGATCATTCTATTTC' | TCGGTTGTTGATGAGATATTTGGA | 55 |
COX 1 | CCCCGCATAAACAACATAAGC | CAGATGCGAGCAGGAGTAGGA | 57 |
ND2 | AAACCCTCGTTCCACAGAAGC' | GGATTATGGATGCGGTTGCT | 58 |
ND5 | GCAGCCATTCAAGCAATCCTA | AGGCGAGGATGAAACCGATA | 60 |
Cyto b | TCGGCATTATCCTCCTGCTT | CTCACGGGAGGACATAGCCTCT | 51 |
Sirt3 | CCCAGTGGCATTCCAGACTT | AAGGGCTTGGGGTTGTGAAA | 55 |
GAPDH | CAACTTTGGTATCGTGGAAGGAC | ACAGTCTTCTGGGTGGCAGTG | 60 |
β-actin | CTGGCACCCAGCACAATG | GTTCATGAGGCACACCTAGCC | 55 |
18s rRNA | GAGTATGGTTGCAAAGCTGAAACTT | TGAGTCAAATTAAGCCGCAGG | 58 |
