Figure 3

Western blot analysis of NALP1 protein expression in 33 human colorectal normal and cancer tissues. (a) The total soluble protein lysates (25 μg) were separated by SDS-PAGE and analyzed for protein expression. The sample information is described in Table 3. For example, ‘N’ and ‘C’ under the heading ‘Patient 3’ are represented as CL3N and CL3T in Supplementary Table 2, respectively. β-Actin protein expression served as a loading control. (b) The human colorectal cancer tissue western blot analysis was used to determine expression levels of NALP1 protein. For the human tissue array, tissues were selected from 33 matched pairs of normal and cancer tissues. The y axis represents the IOD value of NALP1 protein. Data were obtained using the Gel-Pro32 analyzer software with the values normalized to β-actin levels. The bar with the single sign (^) indicates that the expression value of NALP1 protein in cancer tissue is higher than normal tissue. (c) The expression levels of NALP1 protein in human colorectal normal and cancer tissue, respectively. The western analysis was used to determine the expression levels of NALP1 protein. The y axis represents the integrated optical density (IOD) value of NALP1 protein expression. Data were obtained using the Gel-Pro32 analyzer software with the values normalized to β-actin levels. The difference in the expression level of NALP1 protein in colorectal normal versus cancer tissue was highly statistically significant as determined by Wilcoxon signed-rank nonparametric test; the P-values are represented by asterisks (**P<0.01)