Figure 5
From: DAC can restore expression of NALP1 to suppress tumor growth in colon cancer

Expression of NALP1 in colon cancer cell lines before and after DAC treatment. (a) The cytotoxicity of DAC in vitro. No cytotoxicity was observed in any of the three cell lines at 1.0 μM DAC. (b) Expression of NALP1 mRNA relative to GAPDH in three kinds of colon cancer cell lines before and after DAC treatment. (c) Western blot analysis of NALP1 protein before and after DAC treatment. (d) Representative immunofluorescence staining of NALP1 in LS174T cells (untreated with DAC, left panel; treated with DAC, right panel)