Figure 2
NSC697923 inhibits NB cells colony-formation ability. (a) A panel of six NB cell lines were seeded in six-well plates at 102/well, respectively, and allowed to grow for 24 h. Cells were then incubated with indicated concentrations of NSC697923 and grown in RPMI 1640 medium for 2 weeks. The colonies produced were fixed, stained with crystal violet dye, and photographed. (b) A panel of six NB cell lines were seeded in six-well plates with NSC697923 and agar, and grown for 2 weeks. Cells were stained with MTT for 4 h, pictures were taken and colonies were counted. Colony numbers were represented as mean±S.D. P-values <0.01 (**) or <0.001 (***) were indicated. Data from a to b are representative of three independent experiments
