Figure 2 | Cell Death & Disease

Figure 2

From: Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate

Figure 2

Primary ccRCC cells show little sensitivity to respiratory inhibition. (a) Electron microscope image from a ccRCC tissue sample. Note the large amount of lipid droplets (L) and glycogen deposits (*) in the cytoplasm, and also the apparent absence of mitochondria. N=nucleus. (b) The mean values of the relative basal OCR in cultured primary ccRCC cells (R103T, R105T, R110T, R130T and R138T) compared with normal kidney epithelial cells (R103N, R104N and R105N). OCR was determined using the Seahorse technique, data were normalized to number of cells. (c) Seahorse experiment showing the low maximal respiratory capacity after addition of FCCP in the same amount of cells of the primary R105T ccRCC culture compared with the R105N normal kidney epithelial cells. Note the very low basal OCR level of the primary ccRCC cells. (d) WST-1 assay showing the high tolerance of primary ccRCC cells to inhibition of mitochondrial respiration by treatment with As2O3 for 3 days, as compared with normal kidney epithelial cells. Data represent the mean values from two different normal and ccRCC samples (R104N, R117N and R111T, R120T, respectively)

Back to article page