Figure 3 | Cell Death & Disease

Figure 3

From: Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate

Figure 3

3BrPA inhibits growth of primary ccRCC cells. WST-1 assay showing the effect of 50 μM 3BrPA on five different cultures of primary ccRCC (a) and four cultures of normal kidney cells (b), after 3 days of treatment. (c) Viability determined by nucleocounter of primary cultured cells from four normal or ccRCC samples after incubation for 24 h with increasing concentration of 3BrPA. (d and e) Relative number of dead cells determined by nucleocounter after 24 h treatment with the indicated concentrations of 3BrPA in ccRCC (d) or normal (e) cells. (f) Percentage apoptotic (Annexin V and Propidium Iodide-positive) ccRCC cells in control (left) or after 24 h treatment with 50 μM 3BrPA (right). Results from one representative experiment are shown

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