Figure 4 | Cell Death & Disease

Figure 4

From: Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate

Figure 4

3BrPA inhibits lactate excretion and ATP production in primary ccRCC cells. (a) Lactate levels in medium from primary ccRCC cultures were measured after 1-h treatment with 50 or 100 μM 3BrPA. Data presented as relative lactate levels compared with medium from untreated cells. Data shown are an average of results from nine different tumor samples (R110T, R111T, R122T, R132T, R133T, R135T, R138T, R139T and R140T). (b) Intracellular ATP levels in primary ccRCC cells were analyzed by a luciferase assay after 1-h treatment with 50 or 100 μM 3BrPA. Data shown are an average of results from 10 different tumor samples (R110T, R111T, R122T, R130T, R132T, R133T, R135T, R138T, R139T and R140T)

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