Figure 5 | Cell Death & Disease

Figure 5

From: Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate

Figure 5

Established cell lines are less sensitive to glycolytic inhibition. (a) Effect on growth of the established ccRCC cell lines 786-O and WT7 after treatment with 50 μM 3BrPA for 24 h as determined by WST-1 assay. (b) Dose–response curve showing the average viability of seven established ccRCC cell lines (786-O, WT7, SKRC7, SKRC10, SKRC17, SKRC21 and SKRC52) compared with average from four cultured primary normal samples (R132N, R135N, R137N and R138N) or ccRCC cells (R132T, R122T, R138T and R143T) after 24-h treatment with increasing concentrations of 3BrPA, determined using the Nucleocounter. (c) Relative amount of dead cells from the same experiment as in b, after 24-h treatment with indicated concentrations of 3BrPA. (d) Basal OCR per cell measured by Seahorse in one normal kidney sample, two different primary ccRCC samples, two established ccRCC cell lines (786-O, WT-7) and one breast cancer cell line (MCF7)

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