Figure 1

DRAM1 increases BAX protein levels independent of transcription. (a) A549 cells were treated with 3NP (500 μM) and harvested 24, 48 and 72 h later. Bars represent mean±S.E.; n=4; **P<0.01 versus 0 h; ^##P<0.01 versus 0 h. (b) A549 cells were transfected with DRAM1 small interfering RNA (siRNA) or a non-silencing siRNA for 24 h. Cells were then treated with or without 3NP (500 μM) for another 24 h. Bars represent mean±SE; n=4; **P<0.01 versus NC; ^#P<0.05; ^##P<0.01 v ersus NC; ^$$P<0.01 versus NC+3NP; ^&&P<0.01 ve rsus NC+3NP. (c) HeLa cells were transfected with a vector or DRAM1 plasmid for 48 h. Bars represent mean±S.E.; n=4; **P<0.01 versus vector; ^##P<0.01 v ersus vector. (d) HeLa cells were treated with 3NP (500 μM) for 48 h. RNAs were isolated and amplified with qRT-PCR. Bars represent mean±S.E.; n=4; **P<0.01 versus control. (e) HeLa cells were transfected with a vector or DRAM1 plasmid for 48 h. RNAs were isolated and amplified with qRT-PCR. Bars represent mean±S.E.; n=4; ***P<0.001 versus vector. NC, negative control