Figure 2

BAX is degraded by autophagy and DRAM1 interferes with BAX degradation. (a and b) Cells were treated with UPS inhibitor MG132 (40 μM) for 1, 2, 4 and 6 h or lactacystin (4 μM) for 2, 4, 6, and 9 h. The cell lysates were subjected to immunoblotting. β-Actin served as a loading control and Bcl-2 served as a positive control. Bars represent mean±S.E.; n=3. (c) HeLa cells were treated with an autophagy inhibitor 3MA (1.5 mg/ml) for 1, 2, 4 and 6 h. Bars represent mean±S.E.; n=4; *P<0.05; **P<0.01 versus 0 h. (d) HeLa cells were treated with an autophagy inhibitor chloroquine (20 μM) for 2, 4, 6 and 12 h. Bars represent mean±S.E.; n=4; *P<0.05; **P<0.01 versus 0 h. (e) Cells were transfected with Atg5 small interfering RNA (siRNA) for 48 h to inhibit autophagy. Bars represent mean±S.E.; n=4; *P<0.05; **P<0.01 versus NC. NC, negative control; 3MA, 3-methyladenine