Figure 5
From: DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX
BAX mediates the proapoptotic role of DRAM1. (a) A549 cells were treated with 3NP (500 μM) and harvested 24, 48 and 72 h later for the preparation of mitochondrial and cytosolic fractions. Bars represent mean±S.E.; n=3; **P<0.01 versus 0h. (b) A549 cells were transfected with DRAM1 small interfering RNA (siRNA) or non-silencing (Non-sil) siRNA for 24 h and then treated with 3NP (500 μM) for 24 h. Bars represent mean±S.E.; n=3; **P<0.01 versus NC; #P<0.05; ##P<0.01 versus 3NP+NC. (c) A549 cells were transfected with BAX siRNA or Non-sil siRNA for 24 h and then treated with 3NP (500 μM) for 24 h. Bars represent mean±S.E.; n=3; **P<0.01 versus NC; ##P<0.01 v ersus 3NP+NC. NC, negative control
