Figure 6

BAX mediates proapoptotic role of DRAM1 partially through tBID. (a) Immunoblot analysis of the activation of caspase-3. A549 cells were treated with 3NP (500 μM) for 24, 48 and 72 h; the activation of caspase-3 was determined by immunoblotting. Bars represent mean±S.E.; n=3; *P<0.05, **P<0.01 versus 0 h. (b) A549 cells were transfected with DRAM1 small interfering RNA (siRNA) or non-silencing (Non-sil) siRNA for 24 h and then treated with 3NP (500 μM) for 24 h. Bars represent mean±S.E.; n=3, **P<0.01 versus NC; ^#P<0.05, ^##P<0.01 v ersus 3NP+NC. (c) A549 cells were transfected with BAX siRNA or Non-sil siRNA for 24 h and then treated with 3NP (500 μM) for 24 h. Bars represent mean±S.E.; n=3, **P<0.01 versus NC; ^##P<0.01 versus 3NP+NC. (d) Efficiency of siRNA-mediated downregulation of BID. (e) A549 cells were transfected with BID siRNA or Non-sil siRNA for 24 h and then treated with 3NP (500 μM) for another 24 h. Active caspase-3 was determined by immunoblotting. Bars represent mean±S.E.; n=3; *P<0.05 versus NC, ^#P<0.05 versus NC+3NP. NC, negative control